Seratec AmylasePaper
Rapid and sensitive localisation of latent saliva stains on virtually any surface or substrate. Enables large-area screening for salivary alpha-amylase without consuming sample material. For forensic use only.
Large-Area Saliva Stain Localisation
Seratec AmylasePaper enables rapid, sensitive localisation of latent saliva stains across large surface areas — fabric, clothing, bedding, upholstery, and other forensic substrates — without the need to destructively sample the item first. The filter paper is impregnated with a starch-iodine reagent system that reacts with salivary alpha-amylase activity to produce a visible colour change at the site of saliva deposition.
AmylasePaper is used as a pre-screening tool to identify areas of interest on an exhibit before targeted sampling for DNA analysis or immunochromatographic confirmation. This approach preserves sample material and focuses forensic resources on areas most likely to yield probative DNA evidence.
The product is available in A4 format (Cat. No. AMY-PS/25) for routine casework use and in A2 format (Cat. No. AMY-PA2) for screening larger exhibits. Both formats achieve a sensitivity of 125 mIU/mL of amylase, equivalent to a 1:2,000 dilution of human saliva.
Manufacturer: Seratec — seratec.com · Australian Distributor: Advanced Biotech Pty Ltd
Available Formats
AmylasePaper is available in two formats to suit different exhibit sizes and casework requirements.
AmylasePaper A4 — Cat. No. AMY-PS/25
Box with 25 filter paper sheets (A4 size) and 50 mL of Potassium Iodide stock solution. Designed for routine forensic casework screening of standard-sized exhibits including garments, bedding sections, and other fabric items.
Sensitivity: 125 mIU/mL of amylase
Contact Advanced Biotech Pty Ltd for pricing and availability in Australia.
AmylasePaper A2 — Cat. No. AMY-PA2
Filter paper sheet in A2 format for screening larger exhibits. Packaged in boxes of 10 or 100 sheets. Customised packaging available for high-volume laboratory requirements.
Sensitivity: 125 mIU/mL of amylase
Contact Advanced Biotech Pty Ltd for pricing, pack size options, and availability in Australia.
How to Use AmylasePaper
AmylasePaper follows a straightforward contact-transfer workflow compatible with routine forensic evidence examination procedures.
Exhibit Receipt & Documentation
Receive and log the forensic exhibit per laboratory chain-of-custody procedures. Document the item condition and identify the area or areas of interest to be screened for saliva.
Paper Preparation
Prepare the AmylasePaper by moistening it with the supplied Potassium Iodide stock solution as specified in the Instructions for Use. Ensure even and adequate moistening of the paper surface prior to contact with the exhibit.
Contact Application to Exhibit
Place the moistened AmylasePaper directly onto the surface of the exhibit. Apply even pressure across the paper to ensure consistent contact between the paper and the substrate being screened.
Enzymatic Reaction
Salivary alpha-amylase present in the stain transfers to the paper and hydrolyses the starch in the reagent system. This disrupts the starch-iodine complex and produces a visible clearing or colour change at the site of amylase activity within approximately 10 minutes.
Result Interpretation & Area Marking
Identify areas of clearing or colour change on the paper as indicative of amylase activity. These areas correspond to regions of potential saliva deposition on the exhibit. Mark or photograph the exhibit to document areas of interest for targeted follow-up sampling.
Targeted Sampling for Confirmation & DNA
Collect targeted samples from identified areas of interest for further analysis — immunochromatographic confirmation using the Seratec AmylaseTest or PAM Test, and/or DNA extraction and profiling. Document all screening results in the case file per SOP.
Applications
Forensic settings and exhibit types where AmylasePaper is used for saliva stain localisation and evidence triage.
Clothing & Fabric Screening
Rapid screening of garments, underwear, and bedding for saliva stains in sexual assault casework — enabling targeted sampling of positive areas rather than indiscriminate cutting of the entire exhibit.
Sexual Assault Casework
Localisation of oral contact areas on clothing and other fabric exhibits to guide DNA triage and sampling priorities, maximising the value of exhibits submitted for body fluid analysis.
Bite Mark Evidence
Screening of fabric or skin areas adjacent to bite marks to localise salivary alpha-amylase deposition from the bite, supporting targeted swabbing and subsequent DNA profiling.
Large Exhibit Screening
Efficient screening of large fabric items — bedsheets, clothing, upholstery — using A2-format paper to cover greater surface areas in a single application, improving screening throughput.
Pre-Screening Before Immunochromatographic Testing
Used as a first-pass mapping tool prior to confirmatory testing with AmylaseTest or PAM Test — focusing immunochromatographic resources on areas most likely to yield a positive result.
DNA Evidence Triage
Identification of saliva-positive areas on exhibits to prioritise DNA extraction, preserving sample material and laboratory resources for the most probative exhibit regions.
Key Benefits
Any Surface or Material
Applicable to any type of fabric or material — clothing, bedding, upholstery, paper, and other forensic substrates — without restriction by colour or texture.
Fast Results — Under 10 Minutes
Visible colour change at sites of amylase activity within approximately 10 minutes, enabling rapid saliva localisation across large exhibit areas without lengthy incubation.
High Sensitivity
Limit of detection of 125 mIU/mL of amylase — equivalent to a 1:2,000 dilution of human saliva — enabling detection of trace and diluted saliva deposits.
More Specific Than ALS
Provides greater specificity than alternative light source (ALS) fluorescence screening — particularly useful on dark-coloured clothing where ALS performance is limited.
DNA Compatible
Sample material on the exhibit remains suitable for DNA analysis after AmylasePaper screening — the contact transfer method does not consume or degrade the exhibit.
Compatible with Downstream Testing
Fully compatible with subsequent semen and blood testing on the same exhibit, supporting parallel or sequential body fluid screening workflows.
Technical Specifications
| Product name | Seratec AmylasePaper |
|---|---|
| Detection method | Colorimetric starch-iodine enzymatic activity assay |
| Target analyte | Salivary alpha-amylase (enzymatic activity) |
| Biological marker for | Saliva |
| Sensitivity | 125 mIU/mL of amylase (1:2,000 dilution of human saliva) |
| Result time | Less than 10 minutes |
| Available formats | A4 (AMY-PS/25) and A2 (AMY-PA2) |
| Pack size — A4 | Box of 25 sheets with 50 mL Potassium Iodide stock solution (Cat. No. AMY-PS/25) |
| Pack size — A2 | Boxes of 10 or 100 sheets; customised packaging available (Cat. No. AMY-PA2) |
| Substrate compatibility | Any fabric or material type including dark-coloured substrates |
| DNA compatibility | Sample material remains suitable for DNA analysis post-screening |
| Regulatory category | Forensic — for forensic use only |
| Manufacturer | Seratec |
| Australian distributor | Advanced Biotech Pty Ltd |
Downloads
Product documentation for Seratec AmylasePaper. Contact Advanced Biotech to request further technical documentation.
Seratec AmylasePaper is a forensic screening tool. For forensic use only. This product is not approved or intended for clinical diagnostic use. Results must be interpreted by qualified forensic scientists or laboratory personnel operating within accredited forensic laboratory environments and in accordance with validated standard operating procedures.
AmylasePaper is a presumptive screening test. Positive areas should be confirmed with an immunochromatographic assay per laboratory SOP. Australian Distributor: Advanced Biotech Pty Ltd — admin@advancedbiotech.com.au — 0413 654 860.
Frequently Asked Questions
Common questions about Seratec AmylasePaper for forensic saliva stain localisation.
AmylasePaper is used for the rapid localisation of latent saliva stains on forensic exhibits including clothing, fabric, bedding, and other surfaces. It detects salivary alpha-amylase enzymatic activity via a colorimetric starch-iodine reaction, producing a visible colour change at sites of saliva deposition to guide targeted sampling for DNA analysis or confirmatory testing. For forensic use only.
No. The contact transfer method used with AmylasePaper does not consume sample material from the exhibit. Sample material on the exhibit remains suitable for DNA analysis after screening, and the technique is fully compatible with subsequent semen and blood testing on the same exhibit.
Both the A4 and A2 formats of AmylasePaper achieve a sensitivity of 125 mIU/mL of amylase, which is equivalent to a 1:2,000 dilution of human saliva. This allows detection of trace and diluted saliva deposits across a range of forensic substrate types.
AmylasePaper provides greater specificity for saliva than alternative light source (ALS) fluorescence screening, particularly on dark-coloured fabrics where ALS performance is limited. While ALS detects a broad range of biological fluids and fibres through fluorescence, AmylasePaper detects amylase enzymatic activity specifically associated with saliva, reducing non-specific positive areas on the exhibit.
AmylasePaper is available in two formats: A4 format (Cat. No. AMY-PS/25) — box of 25 sheets with 50 mL Potassium Iodide stock solution; and A2 format (Cat. No. AMY-PA2) — boxes of 10 or 100 sheets with customised packaging available. Contact Advanced Biotech Pty Ltd for pricing and availability in Australia.
Seratec AmylasePaper is distributed in Australia by Advanced Biotech Pty Ltd. Contact us to request pricing, pack sizes, availability, and product documentation including the Instructions for Use and Safety Data Sheet.
Enquire About AmylasePaper
Contact Advanced Biotech Pty Ltd to request pricing, pack sizes, availability, and technical documentation for Seratec AmylasePaper in Australia.
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