Seratec PAM Test
Multiplex immunochromatographic lateral flow assay for simultaneous forensic detection of multiple body fluid markers in a single test. For forensic use only.
Forensic Multi-Fluid Detection
The Seratec PAM Test is a multiplex immunochromatographic lateral flow assay for the simultaneous presumptive identification of multiple body fluid markers from a single stain extract. By incorporating multiple antibody zones on a single lateral flow membrane, each targeting a distinct body fluid marker, the PAM Test enables detection of combinations of blood (hemoglobin), semen (PSA), saliva (salivary alpha-amylase), and menstrual blood markers in a single test run.
Mixed stains containing multiple body fluids are common in sexual assault and violent crime casework. Traditional single-fluid assays require separate tests for each body fluid type, consuming additional extract volume and extending processing time. The multiplex approach of the PAM Test enables faster preliminary characterisation of complex stains, supporting exhibit triage and casework prioritisation in high-volume forensic laboratory environments.
Each marker line on the PAM Test device is read independently — the combination of positive lines indicates which body fluids are present in the extract. Results are available within approximately 10 minutes of sample application, without specialist instrumentation.
Manufacturer: Seratec — seratec.com · Australian Distributor: Advanced Biotech Pty Ltd
Intended Use
Defined scope of application for the Seratec PAM Test in professional forensic laboratory settings.
Multiplex Forensic Body Fluid Identification
The Seratec PAM Test is intended for the simultaneous presumptive identification of multiple body fluid markers in biological stain extracts recovered from crime scene evidence, sexual assault examination kits, reference samples, and other forensic materials submitted for body fluid analysis.
The test simultaneously targets markers for blood (hemoglobin), semen (PSA/p30), saliva (salivary alpha-amylase), and menstrual blood in a single lateral flow run. Each marker zone is read independently, enabling preliminary characterisation of mixed stains from a single extract aliquot.
The PAM Test is intended for use by trained forensic scientists and laboratory personnel operating within accredited forensic laboratory environments, in accordance with validated standard operating procedures.
Scope & Limitations
Indicated for: Forensic stain extract testing for simultaneous multi-fluid body fluid presumptive identification. Compatible with dried stains from fabric, swabs, paper, and other forensic substrates following validated extraction procedures.
Result interpretation: Each marker result is independent. A positive line for one marker does not confirm or exclude another. Results must be interpreted collectively in the context of all available casework information and per laboratory SOP.
Not indicated for: Clinical diagnostic use. Not for use as a standalone confirmatory test — positive results may require confirmatory single-fluid testing as appropriate per casework requirements and laboratory SOP. For forensic use only.
Test Workflow
The PAM Test follows a standard lateral flow immunochromatographic workflow, compatible with routine forensic evidence processing procedures.
Evidence Receipt & Stain Identification
Receive and log the forensic exhibit per laboratory chain-of-custody procedures. Identify the stain area of interest on the substrate — fabric cutting, swab, or other evidence item — for targeted extraction. Document the presumed stain location and composition prior to sampling.
Stain Extract Preparation
Prepare the stain extract by eluting the substrate in the extraction buffer as specified in the product Instructions for Use. Ensure the extract volume and dilution are within the validated range for all target markers prior to test application. Note that single extract aliquots may contain markers from more than one body fluid in mixed stains.
Sample Application
Apply the prepared stain extract to the sample well of the PAM Test lateral flow device. Ensure the correct volume is applied as specified in the IFU. Place the device on a flat, level surface and do not move or disturb during test migration.
Multiplex Lateral Flow Migration
The sample migrates across the nitrocellulose membrane by capillary action. Each body fluid marker present in the extract binds to its corresponding antibody conjugate and is captured at its specific test line zone on the membrane, producing a visible line independently of other marker zones. The control line confirms adequate sample flow and reagent integrity across the entire membrane.
Multi-Marker Result Interpretation
Read all marker lines simultaneously at the time point specified in the IFU (approximately 10 minutes). Each visible marker line is recorded independently — any visible line at a marker zone constitutes a positive for that body fluid marker. The pattern of positive lines is interpreted collectively to characterise the body fluid composition of the extract. An invalid result (no control line) requires repeat testing. Refer to the IFU for the complete multi-marker interpretation guide.
Documentation & Chain of Custody
Record all marker results, the test device lot number, and read time in the laboratory case file per SOP. Document the pattern of positive and negative marker lines. Positive results may be submitted for confirmatory single-fluid analysis and downstream DNA profiling as indicated by casework requirements and laboratory standard operating procedures.
Multi-Fluid Result Interpretation
Each marker zone on the PAM Test is read independently. The combination of positive and negative marker lines collectively characterises the body fluid composition of the extract.
Individual Marker Positive
A visible line at a specific marker zone indicates that body fluid marker is detected in the extract above the assay threshold. The line may appear at any intensity — any visible line constitutes a positive for that marker. The control line must be visible for the result to be valid. Record each positive marker independently per IFU.
No Markers Detected
All marker lines are absent and the control line is present. Indicates that none of the target body fluid markers were detected in the extract above the assay detection threshold. A negative result across all markers reduces but does not exclude the presence of body fluids below the assay detection limit. Document per SOP.
Mixed Stain
Multiple marker lines are visible simultaneously, indicating the presence of more than one body fluid marker in the extract — consistent with a mixed stain. Each positive marker is interpreted independently. The combination of positive lines provides a preliminary characterisation of the body fluid composition. Confirmatory single-fluid testing should be conducted as appropriate per laboratory SOP.
Applications
Forensic settings and casework contexts where the PAM Test is used for multi-fluid body fluid identification and evidence triage.
Sexual Assault Casework
Primary application for simultaneous multi-fluid screening in sexual assault examination kits — enabling rapid preliminary characterisation of mixed stains on swabs, clothing, and bedding from a single extract aliquot, supporting triage and DNA prioritisation.
Complex & Mixed Stain Analysis
Forensic analysis of exhibits where multiple body fluid types may be co-deposited. The multiplex format characterises the body fluid composition of complex stains in a single test run, reducing the number of sequential single-fluid tests required.
Violent Crime Investigation
Screening of crime scene evidence including clothing, weapons, and surfaces in assault and homicide investigations where multiple body fluids may be present, enabling faster preliminary fluid type characterisation for investigative and DNA triage purposes.
Exhibit Triage in High-Volume Laboratories
Rapid multi-fluid screening of high-volume exhibit backlogs enables laboratory scientists to identify and prioritise exhibits containing forensically significant body fluid combinations without running multiple sequential single-fluid assays per exhibit.
Forensic Reference Laboratories
Accredited forensic reference laboratories requiring a validated, standardised multiplex rapid test for simultaneous body fluid identification in routine and complex casework, supporting workflow efficiency and exhibit characterisation reporting.
Cold Case Evidence Re-examination
Re-examination of archived forensic evidence for multiple body fluid markers, supporting cold case investigations where historical samples require multi-fluid characterisation from conserved extract volumes.
Technical Specifications
| Product name | Seratec PAM Test |
|---|---|
| Test format | Multiplex lateral flow immunochromatographic assay (LFA) |
| Detection format | Multiplex qualitative — simultaneous multi-marker detection |
| Target markers | Multiple body fluid markers simultaneously, including combinations of: hemoglobin (blood), PSA/p30 (semen), salivary alpha-amylase (saliva), and menstrual blood markers (refer to IFU for full marker panel) |
| Body fluids targeted | Blood, semen, saliva, menstrual blood (multiplex — refer to IFU for specific marker panel) |
| Sample type | Biological stain extract prepared per IFU |
| Substrate compatibility | Fabric, swabs, paper, dried biological deposits (refer to IFU for validated substrates) |
| Result time | Approximately 10 minutes (refer to IFU for validated read window) |
| Result interpretation | Visual — multiple independent marker lines plus control line on a single lateral flow membrane |
| Positive marker result | Visible line at a marker zone (any intensity) in presence of valid control line |
| Negative marker result | No visible line at a marker zone; control line present |
| Invalid result | No control line visible — repeat test required |
| Mixed stain result | Multiple marker lines visible simultaneously — each interpreted independently |
| Storage temperature | 2–30°C (refer to product label and IFU) |
| Shelf life | Refer to product label and IFU |
| Regulatory category | Forensic — for forensic use only |
| Manufacturer | Seratec |
| Australian distributor | Advanced Biotech Pty Ltd |
| Contact | admin@advancedbiotech.com.au · 0413 654 860 |
Downloads
Product documentation for the Seratec PAM Test. Contact Advanced Biotech to request the Instructions for Use and Safety Data Sheet.
The Seratec PAM Test is a forensic diagnostic test. For forensic use only. This product is not approved or intended for clinical diagnostic use. Results must be interpreted by qualified forensic scientists or laboratory personnel operating within accredited forensic laboratory environments and in accordance with validated standard operating procedures.
The PAM Test is a presumptive multiplex test. Each marker result is independent and must be interpreted collectively in the context of all available casework information. A positive marker result does not alone confirm the presence of a body fluid. Confirmatory testing and further forensic analysis should be conducted as appropriate per laboratory SOP and casework requirements.
Australian Distributor: Advanced Biotech Pty Ltd — admin@advancedbiotech.com.au — 0413 654 860.
Frequently Asked Questions
Common questions about the Seratec PAM Test forensic multi-fluid detection assay.
The Seratec PAM Test is a multiplex immunochromatographic lateral flow assay that simultaneously detects multiple body fluid markers from a single stain extract. The test uses multiple antibody zones on a single lateral flow membrane, each targeting a distinct body fluid marker. Body fluids that may be detected simultaneously include combinations of blood (hemoglobin), semen (PSA/p30), saliva (salivary alpha-amylase), and menstrual blood markers. Each marker line is read independently. For forensic use only.
The PAM Test uses a single lateral flow membrane that contains multiple distinct antibody zones, each specific for a different body fluid marker. When the stain extract is applied to the device, it migrates across the membrane by capillary action. Each marker present in the extract binds to its corresponding antibody conjugate and is captured at its specific test line zone, producing a visible line if the marker is detected above the threshold concentration. Multiple marker lines may develop independently in the same run, enabling simultaneous presumptive identification of more than one body fluid type from a single application. For forensic use only.
Mixed stains containing multiple body fluids are common in sexual assault and violent crime casework. Traditionally, each body fluid type requires a separate single-fluid test, meaning multiple tests, multiple extract volumes, and extended processing time. The PAM Test enables simultaneous presumptive characterisation of multiple body fluid types from a single stain extract in a single test run, reducing the number of tests required, conserving precious stain extract, and enabling faster preliminary characterisation of complex exhibits during triage. For forensic use only.
Each marker line on the PAM Test is read independently at the time point specified in the Instructions for Use (approximately 10 minutes). A visible line at a marker zone indicates detection of that body fluid marker above the assay threshold. The absence of a visible line indicates that marker was not detected. The control line must be visible for the result to be valid. Invalid result: no control line visible — repeat the test with a new device. Results are interpreted collectively — the combination of positive marker lines indicates which body fluids are present in the extract. Refer to the IFU for the complete multi-marker interpretation guide. For forensic use only.
When multiple marker lines are visible on the PAM Test, this indicates the presence of multiple body fluid markers in the stain extract, consistent with a mixed stain containing more than one body fluid type. Each positive marker line is interpreted independently — a positive line for one marker does not affect the interpretation of another. The pattern of positive lines provides a preliminary characterisation of the body fluid composition of the stain. This result should be interpreted in the context of all available casework information, and confirmatory single-fluid testing should be conducted as appropriate per laboratory SOP. For forensic use only.
The PAM Test is designed for use with biological stain extracts prepared from forensic evidence including fabric cuttings, swabs from sexual assault examination kits, skin swabs, and other substrates where body fluids may be deposited. The stain is eluted in the extraction buffer specified in the Instructions for Use before application to the test device. Refer to the IFU for validated sample preparation procedures and dilution requirements. Note that a single extract aliquot from a mixed stain may yield positive results for more than one marker simultaneously.
The Seratec PAM Test produces results within approximately 10 minutes of sample application. Results for all marker lines are read simultaneously at the time point specified in the Instructions for Use. Do not read results before the minimum validated time or after the specified window has elapsed, as this may affect result accuracy and reliability across all marker zones.
The Seratec PAM Test is distributed in Australia by Advanced Biotech Pty Ltd. Contact us at admin@advancedbiotech.com.au or telephone 0413 654 860 to request pricing, pack sizes, availability, and product documentation including the Instructions for Use and Safety Data Sheet.
Enquire About the PAM Test
Contact Advanced Biotech Pty Ltd to request pricing, pack sizes, availability, and technical documentation for the Seratec PAM Test in Australia.
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